Description
SPRINGER Xenopus Protocols 2006 Edition by LIU, X. J.
A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance, its striking protein translation capacity, and its breathtaking possibilities for the assembly of infectious viral particles by single cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction, and cover all the major uses of oocytes/extracts as experimental models. Resources for Genetic and Genomic Studies of XenopusSteven L. Klein, Daniela S. Gerhard, Lukas Wagner, Paul Richardson, Lynn M. Schriml, Amy K. Sater, Wesley C. Warren, and John D. McPhersonThe Physiology of the Xenopus laevis OvaryMelissa A. Rasar and Stephen R. HammesOocyte Isolation and EnucleationX. Shawn Liu and X. Johne LiuXenopus tropicalis Oocytes: More Than Just a Beautiful GenomeJean-Francois L. Bodart and Nicholas S. DuesberyOocyte Expression With Injection of Purified T7 RNA PolymeraseXavier Altafaj, Nathalie Joux, Michel Ronjat, and Michel De WaardVisualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence MicroscopyBret E. Becker and David L. GardMultiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte ResearchAngela M. Prouty, Jun Wu, Da-Ting Lin, Patricia Camacho, and James D. LechleiterImaging Ca2+ Signals in Xenopus OocytesSheila L. Dargan, Angelo Demuro, and Ian ParkerChromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus EggsAntonin V. Tutter and Johannes C. WalterChromatin Assembly of DNA Templates Microinjected Into Xenopus OocytesDaniele Roche, Genevieve Almouzni, and Jean-Pierre QuivyPre-mRNA Splicing in the Nuclei of Xenopus OocytesKyong Hwa Moon, Xinliang Zhao, and Yi-Tao YuChromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and TadpolesDavid Stewart, Akihiro Tomita, Yun-Bo Shi, and Jiemin WongCytoplasmic mRNA Polyadenylation and Translation AssaysMaria Pique, Jose Manuel Lopez, and Raul MendezXenopus Egg Extracts: A Model System to Study Proprotein ConvertasesKathleen I. J. ShennanAnalysis of Molecular Chaperones Using a Xenopus Oocyte Protein Refolding AssayJohn J. Heikkila,Angelo Kaldis, and Rashid AbdulleUbiquitin-Mediated Protein Degradation in Xenopus Egg ExtractsAnna Castro, Suzanne Vigneron, Cyril Bernis, Jean-Claude Labbe, and Thierry LorcaIntroduction to Nucleocytoplasmic Transport: Molecules and MechanismsReiner PetersUse of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport StudiesReiner PetersNuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force MicroscopyBohumil Maco, Birthe Fahrenkrog, Ning-Ping Huang, and Ueli AebiIn Vitro Study of Nuclear Assembly and Nuclear Import Using Xenopus Egg ExtractsRene C. Chan and Douglass J. ForbesUse of Intact Xenopus Oocytes in Nucleocytoplasmic Transport StudiesNelly PanteStudying the Mechanosensitivity of Voltage-Gated Channels Using Oocyte PatchesCatherine E. Morris, Peter F. Juranka, Wei Lin, Terence J. Morris, and Ulrike LaitkoOocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and PesticidesSteven David Buckingham, Luanda Pym, and David Barry SattelleMicrotransplantation of Neurotransmitter Receptors From Cells to Xenopus Oocyte Membranes: New Procedure for Ion Channel StudiesRicardo Miledi, Eleonora Palma, and Fabrizio EusebiReconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extracts in Semi-Intact MDCK CellsFumi Kano, Katsuya Takenaka, and Masayuki MurataExploring RNA Virus Replication in Xenopus OocytesAndrea V. Gamarnik and Raul AndinoStudy of Apoptosis in Vitro Using the Xenopus Egg Extract Reconstitution SystemPaula Deming and Sally KornbluthStudying Fertilization in Cell-Free Extracts: Focusing on Membrane/Lipid Raft Functions and ProteomicsKen-ichi Sato, Ken-ichi Yoshino, Alexander A. Tokmakov, Tetsushi Iwasaki, Kazuyoshi Yonezawa, and Yasuo