Description
Humana Press Inc. Neutrophil Methods And Protocols by Mark T. Quinn
This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid._x000D_ _x000D_
Neutrophils_x000D_
_x000D_
Methods and Protocols_x000D_
_x000D_
_x000D_
Edited by_x000D_
_x000D_
Mark T. Quinn_x000D_
Frank R. DeLeo_x000D_
Gary M. Bokoch _x000D_
Contents_x000D_
_x000D_
Dedication i_x000D_
Preface ii_x000D_
Contributors v_x000D_
_x000D_
PART I: NEUTROPHILS: AN OVERVIEW_x000D_
_x000D_
1. The Role of Neutrophils in the Immune System: An Overview_x000D_
Harry L. Malech_x000D_
_x000D_
PART II: NEUTROPHIL ISOLATION AND SUBCELLULAR FRACTIONATION_x000D_
_x000D_
2. Isolation of Human Neutrophils From Venous Blood_x000D_
William M. Nauseef_x000D_
_x000D_
3. Neutrophil Isolation From Non-Human Species_x000D_
Daniel W. Siemsen, Igor A. Schepetkin, Lilia N. Kirpotina, Benfang Lei, and Mark T. Quinn_x000D_
_x000D_
4. Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers_x000D_
Lene Udby and Niels Borregaard _x000D_
_x000D_
PART III. BIOCHEMISTRY, BIOLOGY AND SIGNAL TRANSDUCTION OF NEUTROPHILS_x000D_
_x000D_
5. Rac and Rap GTPase Activation Assays_x000D_
Ulla G. Knaus, Alison Bamberg, and Gary M. Bokoch_x000D_
_x000D_
6. Measurement of Phospholipid Metabolism in Intact Neutrophils_x000D_
Susan Sergeant and Linda C. McPhail_x000D_
_x000D_
7. Analysis of Protein Phosphorylation in Human Neutrophils_x000D_
Jamel El-Benna and Pham My-Chan Dang_x000D_
_x000D_
8. Mitogen-Activated Protein Kinase Assays_x000D_
Martine Torres_x000D_
_x000D_
9. Transduction of Proteins into Intact Neutrophils_x000D_
Tieming Zhao and Gary M. Bokoch_x000D_
_x000D_
10. Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils_x000D_
Esther J. Hillson, Sharon Dewitt, and Maurice B. Hallett_x000D_
_x000D_
11. Analysis of Electrophysiological Properties and Responses of Neutrophils_x000D_
Deri Morgan and Thomas E. DeCoursey_x000D_
_x000D_
12. Analysis of Neutrophil Apoptosis_x000D_
Emma L. Taylor, Adriano G. Rossi, Ian Dransfield, and Simon P. Hart_x000D_
_x000D_
PART IV. NEUTROPHIL ADHESION AND CHEMOTAXIS_x000D_
_x000D_
13. Optical and Fluorescence Detection of Neutrophil Integrin Activation_x000D_
Ulrich Y. Schaff, Melissa R. Sarantos, Harold Ting, and Scott I. Simon_x000D_
_x000D_
14. Analysis of Neutrophil Polarization and Chemotaxis_x000D_
Mary A. Lokuta, Paul A. Nuzzi, and Anna Huttenlocher_x000D_
_x000D_
15. Quantifying and Localizing Actin Free Barbed Ends in Neutrophils_x000D_
Michael Glogauer_x000D_
_x000D_
16. Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow_x000D_
Mark A. Jutila, Bruce Walcheck, Robert Bargatze, and Aiyappa Palecanda_x000D_
_x000D_
17. Model Systems to Investigate Neutrophil Adhesion and Chemotaxis_x000D_
Nancy A. Louis, Eric Campbell, and Sean P. Colgan_x000D_
_x000D_
_x000D_
PART V. NEUTROPHIL PHAGOCYTOSIS AND BACTERICIDAL ACTIVITY_x000D_
_x000D_
18. Immunofluorescence and Confocal Microscopy of Neutrophils_x000D_
Lee-Ann H. Allen_x000D_
_x000D_
19. Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy_x000D_
Benjamin E. Steinberg and Sergio Grinstein_x000D_
_x000D_
20. Analysis of Neutrophil Membrane Traffic During Phagocytosis_x000D_
Per Loennbro, Pontus Nordenfelt, and Hans Tapper_x000D_
_x000D_
21. Analysis of Neutrophil Bactericidal Activity_x000D_
Jessie N. Green, Christine C. Winterbourn, and Mark B. Hampton_x000D_
_x000D_
22. A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions_x000D_
Per Follin and Claes Dahlgren_x000D_
_x000D_
PART VI. NADPH OXIDASE AND PRODUCTION OF REACTIVE OXYGEN SPECIES_x000D_
_x000D_
23. Measurement of Respiratory Burst Products Generated by Professional Phagocytes_x000D_
Claes Dahlgren, Anna Karlsson, and Johan Bylund_x000D_
_x000D_
24. The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function_x000D_
Thomas L. Leto, Mark C. Lavigne, Neda Homoyounpour, Kristen Lekstrom, Gilda Linton, Harry L. Malech, and Isabelle de Mendez_x000D_
_x000D_
25. Cell-free Assays: The Reductionist Approach to the Study of NADPH Oxidase Assembly _x000D_
Shahar Molshanski-Mor, Ariel Mizrahi, Yelena Ugolev, Iris Dahan, Yevgeny Berdichevsky, and Edgar Pick_x000D_
_x000D_
26. Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis_x000D_