In Situ Hybridization Protocols by Ian A. Darby , Tim D. Hewitson , Springer

Description

Springer In Situ Hybridization Protocols by Ian A. Darby , Tim D. Hewitson

The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories._x000D_ _x000D_Treatment of Tissue Sections for In Situ Hybridization_x000D_ Gregory H. Tesch, Hui Y. Lan, and David J. Nikolic-Paterson_x000D_ _x000D_ Preparation of Template DNA and Labeling Techniques_x000D_ Peter J. Roche_x000D_ _x000D_ In Situ Hybridization Using cRNA Probes: Isotopic and Nonisotopic Detection Methods_x000D_ Ian A. Darby, Teresa Bisucci, Alexis Desmouliere, and Tim D. Hewitson_x000D_ _x000D_ Tyramide Signal Amplification for DNA and mRNA In Situ Hybridization_x000D_ Ernst J. M. Speel, Anton H. N. Hopman, and Paul Komminoth_x000D_ _x000D_ Expression Analysis of Murine Genes Using In Situ Hybridization With Radioactive and Nonradioactively Labeled RNA Probes_x000D_ Anne Chotteau-Lelievre, Pascal Dolle, and Francoise Gofflot_x000D_ _x000D_ Nonradioactive In Situ Hybridization on Frozen Sections and Whole Mounts_x000D_ Julia Asp, Alexandra Abramsson, and Christer Betsholtz. _x000D_ _x000D_ In Situ Hybridization of Whole-Mount Embryos_x000D_ Murray Hargrave, Josephine Bowles, and Peter Koopman_x000D_ _x000D_ In Situ Detection of Epstein-Barr Virus and Phenotype Determination of EBV-Infected Cells_x000D_ Gerald Niedobitek and Hermann Herbst_x000D_ _x000D_ PNA-In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent Detection of Cellular and Viral Proteins_x000D_ Tomoko Hagiwara, Junko Hattori, and Tsuguhiro Kaneda_x000D_ _x000D_ Electron Microscopy and In Situ Hybridization: Expression of P2Y2 Receptor mRNA in the Cerebellum_x000D_ Andrzej Loesch and Rainer Glass_x000D_ _x000D_ In Situ Hybridization Using Riboprobes on Free-Floating Brain Sections_x000D_ Neil C. Owens, F. Martin Hess, and Emilio Badoer_x000D_ _x000D_ An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells_x000D_ Changsun Choi, Laurie G. Hudson, Pierre Savagner, and Donna F. Kusewitt_x000D_ _x000D_ Identification of Transplanted Human Cells in Animal Tissues_x000D_ Daniel Benten, Kang Cheng, and Sanjeev Gupta_x000D_ _x000D_ In Situ Hybridization to Plant Tissues and Chromosomes_x000D_ AndreasHouben, Sharon J. Orford, and Jeremy N. Timmis_x000D_ _x000D_ Histochemical Localization of Cell Proliferation Using In Situ Hybridization for Histone mRNA_x000D_ Tim D. Hewitson, Kristen J. Kelynack, and Ian A. Darby_x000D_ _x000D_ Histochemical Localization of Apoptosis With In Situ Labeling of Fragmented DNA_x000D_ Tim D. Hewitson, Teresa Bisucci, and Ian A. Darby_x000D_ _x000D_ The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue Sections_x000D_ Malcolm D. Smith, Michael Ahern, and Mark Coleman_x000D_ _x000D_ Semiquantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Motoneuron Populations_x000D_ Paul D. Storer and Tracey DeLucia_x000D_ _x000D_ Quantitative In Situ Hybridization of Tissue Microarrays_x000D_ Adrian M. Jubb, Thinh Q. Pham, Gretchen D. Frantz, Franklin V. Peale, Jr., and Kenneth J. Hillan_x000D_ _x000D_ Index_x000D_